pUC18 DNA Hae III Digest, for DNA electrophoresis

Descriptions

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

pUC18 DNAHaeIII Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

Components

Sigma’;s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

General description

Sigma’;s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Contains 11 fragments, 11–587 bp

Preparation Note

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Pack	Manufacturer	Storage Temp.	Suitability	Form	Price		Quantity
3577369	pUC18 DNA Hae III Digest, for DNA electrophoresis	200, 100	-	SIGMA-ALDRICH	−20°C	suitable for electrophoresis (DNA)	liquid	£654.64 (exc VAT) per EACH		- +
3577370	pUC18 DNA Hae III Digest, for DNA electrophoresis	200, 100	20UG	SIGMA-ALDRICH	−20°C	suitable for electrophoresis (DNA)	liquid	£165.75 (exc VAT) per 20UG		- +