Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondriaDetermination of enzyme localization on membranesTreatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Packaging

1 g in poly bottle

5 mg in glass bottle

10, 25, 100, 500 mg in poly bottle

Unit Definition

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0?µmole of tyrosine per min at pH?7.5 at 37?°C (color by Folin-Ciocalteu reagent).

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Composition Product Line Impurities Form Price Quantity
3581009 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
£309.43 (exc VAT) per 100MG
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3581010 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
£83.74 (exc VAT) per 10MG
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3581011 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
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3581012 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
£97.26 (exc VAT) per 25MG
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3581013 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
£1,023.75 (exc VAT) per 500MG
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3581014 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
£74.66 (exc VAT) per 5MG
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3581015 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, >=30 units/mg protein, for molec 400 SIGMA-ALDRICH Protein, ≥90% biuret BioUltra
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