Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-

Descriptions

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Packaging

500 mg in poly bottle

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 µmole (181 µg) of tyrosine per minute at pH 7.5 at 37°C.

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3581054	Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-	100	SIGMA-ALDRICH	2-8°C	lyophilized powder	£250.71 (exc VAT) per 500MG		- +
3581055	Protease from Bacillus licheniformis, lyophilized powder, for use in Total Dietary Fiber Assay, TDF-	100	SIGMA-ALDRICH	2-8°C	lyophilized powder	£1,037.68 (exc VAT) per EACH		- +