Phytagel, BioReagent, plant cell culture tested, powder

Phytagel, BioReagent, plant cell culture tested, powder

Application

Phytagel™ has been used:in the solidification of culture medium for root assays in Arabidopsis thalianaas a component of Solid Paul?s medium for Agrobacterium cultureas a component of root induction medium in Ilex dumosa tissue culture

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Packaging

100, 250, 500 g in poly bottle

1, 5 kg in poly drum

Reconstitution

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Technique(s) Product Line Form Price Quantity
3582106 Phytagel, BioReagent, plant cell culture tested, powder 200 SIGMA-ALDRICH cell culture - plant: suitable BioReagent powder
£89.00 (exc VAT) per 100G
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3582107 Phytagel, BioReagent, plant cell culture tested, powder 200 SIGMA-ALDRICH cell culture - plant: suitable BioReagent powder
£594.75 (exc VAT) per 1KG
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3582108 Phytagel, BioReagent, plant cell culture tested, powder 200 SIGMA-ALDRICH cell culture - plant: suitable BioReagent powder
£161.57 (exc VAT) per 250G
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3582109 Phytagel, BioReagent, plant cell culture tested, powder 200 SIGMA-ALDRICH cell culture - plant: suitable BioReagent powder
£303.57 (exc VAT) per 500G
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3582110 Phytagel, BioReagent, plant cell culture tested, powder 200 SIGMA-ALDRICH cell culture - plant: suitable BioReagent powder
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