Phospholipase D from Streptomyces sp., Type VII, lyophilized powder, >=150 units/mg solid

Descriptions

Analysis Note

Protein by biuret

Application

Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.

The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.

Biochem/physiol Actions

Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

One unit will liberate 1.0 µmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3581099	Phospholipase D from Streptomyces sp., Type VII, lyophilized powder, >=150 units/mg solid	200	SIGMA-ALDRICH	−20°C	lyophilized powder	Click Add to Quote for price and delivery	- +
3581100	Phospholipase D from Streptomyces sp., Type VII, lyophilized powder, >=150 units/mg solid	200	SIGMA-ALDRICH	−20°C	lyophilized powder	£442.93 (exc VAT) per EACH	- +
3581101	Phospholipase D from Streptomyces sp., Type VII, lyophilized powder, >=150 units/mg solid	200	SIGMA-ALDRICH	−20°C	lyophilized powder	£807.86 (exc VAT) per EACH	- +