Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, >=5,500 DEA

Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, >=5,500 DEA

Analysis Note

Package sizes are based on DEA units

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0

Physical properties

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Unit Definition

One DEA unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37?°C. (One glycine unit is equivalent to ~3 DEA units)

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3578930 Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, >=5,500 DEA 200 SIGMA-ALDRICH 2-8°C buffered aqueous glycerol solution
£284.14 (exc VAT) per EACH
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3578931 Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous glycerol solution, >=5,500 DEA 200 SIGMA-ALDRICH 2-8°C buffered aqueous glycerol solution
£1,137.96 (exc VAT) per EACH
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