Phosphatase, Acid from sweet potato, ammonium sulfate suspension, >=10.0 units/mg protein (modified

Phosphatase, Acid from sweet potato, ammonium sulfate suspension, >=10.0 units/mg protein (modified

Application

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or ?-amylase.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO4 + H2O --------------- R-OH + HOPO3 2+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.

General description

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.

Physical form

Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.3

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

No detailed specifications are available for this product.

There are no downloads for this product.

Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579979 Phosphatase, Acid from sweet potato, ammonium sulfate suspension, >=10.0 units/mg protein (modified 300 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
Click Add to Quote for price and delivery
-
+
3579980 Phosphatase, Acid from sweet potato, ammonium sulfate suspension, >=10.0 units/mg protein (modified 300 SIGMA-ALDRICH 2-8°C ammonium sulfate suspension
£456.86 (exc VAT) per EACH
-
+