PFM01

PFM01

Biochem/physiol Actions

PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 µM), but not the exonuclease inhibitors Mirin (500 µM) and PFM39 (100 µM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed.

Packaging

5, 25 mg in glass bottle

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Code Description Colour Quality Level Manufacturer Storage Temp. Solubility Form Assay Price Quantity
3575322 PFM01 white to beige 100 SIGMA-ALDRICH 2-8°C
£536.25 (exc VAT) per 25MG
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3575323 PFM01 white to beige 100 SIGMA-ALDRICH 2-8°C
£150.43 (exc VAT) per 5MG
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