Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg

Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Other Notes

Sales restrictions may apply

Unit Definition

1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat.No. 51290, as substrate) 15′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U. 1 Bergmeyer-U is the amount of enzyme which hydrolyzes 1 μmol Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C

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Code Description Colour Manufacturer Storage Temp. Form Price Quantity
3578879 Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg slightly beige SIGMA-ALDRICH 2-8°C powder
£105.02 (exc VAT) per 1G
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3578880 Pepsin from porcine gastric mucosa, powder, slightly beige, 1200-2400 U/mg slightly beige SIGMA-ALDRICH 2-8°C powder
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