o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet

Descriptions

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 µl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	mp	InChI key	InChI	Manufacturer	Storage Temp.	Solubility	Form	Price		Quantity
3578682	o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet	200	258 °C (dec.) (lit.)	RIIWUGSYXOBDMC-UHFFFAOYSA-N	1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H	SIGMA-ALDRICH	2-8°C	water: 1 tablet/10 mL, clear, colorless	tablet	£426.21 (exc VAT) per EACH		- +
3578683	o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet	200	258 °C (dec.) (lit.)	RIIWUGSYXOBDMC-UHFFFAOYSA-N	1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H	SIGMA-ALDRICH	2-8°C	water: 1 tablet/10 mL, clear, colorless	tablet	£247.93 (exc VAT) per EACH		- +