Nitrotetrazolium Blue chloride, powder, for molecular biology

Application

Nitrotetrazolium Blue chloride has been used: to determine the superoxide anion in skeletal muscle cells as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sectionsas a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlingsas a component in Triton X-100 solution to perform staining of coronal brain sections

Biochem/physiol Actions

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2.

General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Packaging

1 g in glass bottle

50, 250 mg in glass insert

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Code Description Quality Level InChI key InChI Manufacturer Solubility Form Assay Price Quantity
3582065 Nitrotetrazolium Blue chloride, powder, for molecular biology 100 FSVCQIDHPKZJSO-UHFFFAOYSA-L 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8- SIGMA-ALDRICH
£240.96 (exc VAT) per 1G
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3582066 Nitrotetrazolium Blue chloride, powder, for molecular biology 100 FSVCQIDHPKZJSO-UHFFFAOYSA-L 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8- SIGMA-ALDRICH
£125.36 (exc VAT) per 250MG
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3582067 Nitrotetrazolium Blue chloride, powder, for molecular biology 100 FSVCQIDHPKZJSO-UHFFFAOYSA-L 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8- SIGMA-ALDRICH
£58.64 (exc VAT) per 50MG
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