MUTANOLYSIN FROM STREPTOMYCES GLOBISPORU

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

Legal Information

Ficoll is a registered trademark of Cytiva

Packaging

25000, 100000 units in poly bottle

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Unit Definition

One unit will produce a δA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3581351 MUTANOLYSIN FROM STREPTOMYCES GLOBISPORU 400 SIGMA-ALDRICH −20°C lyophilized powder
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3581352 MUTANOLYSIN FROM STREPTOMYCES GLOBISPORU 400 SIGMA-ALDRICH −20°C lyophilized powder
£675.54 (exc VAT) per EACH
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