MTT Formazan, MTT reduction end product

MTT Formazan, MTT reduction end product

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

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Code Description Quality Level InChI key InChI Manufacturer Solubility Form Price Quantity
3578019 MTT Formazan, MTT reduction end product 100 GIFKVQOPAJDJLO-SZVLODPRSA-N 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+ SIGMA-ALDRICH chloroform: 10 mg/mL, clear, dark violet powder
£181.07 (exc VAT) per 1G
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3578020 MTT Formazan, MTT reduction end product 100 GIFKVQOPAJDJLO-SZVLODPRSA-N 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+ SIGMA-ALDRICH chloroform: 10 mg/mL, clear, dark violet powder
£59.06 (exc VAT) per 250MG
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