Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, b

Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, b

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.Western Blotting and EIABrowse additional application references in our FLAG® Literature portal.

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

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Code Description Manufacturer Form Price Quantity
3586935 Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, b SIGMA-ALDRICH buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Click Add to Quote for price and delivery
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3586936 Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, b SIGMA-ALDRICH buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
£199.50 (exc VAT) per 50UG
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3586937 Monoclonal ANTI-FLAG M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, b SIGMA-ALDRICH buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Click Add to Quote for price and delivery
-
+