Minimum Essential Medium Eagle, With Earles salts, non-essential amino acids and sodium bicarbonate,

Minimum Essential Medium Eagle, With Earles salts, non-essential amino acids and sodium bicarbonate,

Application

Minimum Essential Medium Eagle has been used to culture:HeLa cellsMadin-Darby canine kidney (MDCK) cellschondrosarcoma cell lineThis medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

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Code Description Quality Level Pack Manufacturer Technique(s) Storage Temp. Impurities Form Price Quantity
3577111 Minimum Essential Medium Eagle, With Earles salts, non-essential amino acids and sodium bicarbonate, 400 1L SIGMA-ALDRICH cell culture - mammalian: suitable
£75.81 (exc VAT) per 1L
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3577112 Minimum Essential Medium Eagle, With Earles salts, non-essential amino acids and sodium bicarbonate, 400 500ML SIGMA-ALDRICH cell culture - mammalian: suitable
£39.93 (exc VAT) per 500ML
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