Meridian Bioscience Ribonuclease Inhibitor (RiboSafe RNase Inhibitor) is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C by binding non-covalently in a 1:1 ratio (Fig. 1 & 2). RiboSafe shows no inhibition of polymerase or transcriptase activity (Fig. 4) and it is not effective against RNase H, T1, S1-nuclease or RNase from Aspergilus. With an association constant of 1014M, RiboSafe is useful in any applications where the presence of RNases is a potential problem. RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases and exonucleases. The enzyme is supplied at a concentration of 40u/µl.
Features
Complete inhibition of RNase A, B and C
Significantly increases RT-PCR sensitivity
DNase/RNase and Nickase free
No inhibition of polymerase/transcriptase activity
Stable over a wide range of pH, temperatures and DTT concentrations
Applications
RNA purification
cDNA preparation by reverse transcription
RNA sequencingin vitro RNA transcription
in vitro protein synthesis
Storage Conditions RiboSafe RNase Inhibitor can be stored for 6 months at -20°C.