Malic Dehydrogenase from porcine heart, >=600 units/mg protein (biuret), ammonium sulfate suspension

Descriptions

Application

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

Malic Dehydrogenase from porcine heart has been used:in qualitative protein binding measurementsto test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pairto investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Biochem/physiol Actions

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH⁺. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

General description

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.

Physical form

Suspension in 2.8 M (NH₄)₂SO₄ solution, pH 6.0

Unit Definition

One unit will convert 1.0 µmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579861	Malic Dehydrogenase from porcine heart, >=600 units/mg protein (biuret), ammonium sulfate suspension	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£211.71 (exc VAT) per EACH	- +
3579862	Malic Dehydrogenase from porcine heart, >=600 units/mg protein (biuret), ammonium sulfate suspension	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£532.07 (exc VAT) per EACH	- +
3579863	Malic Dehydrogenase from porcine heart, >=600 units/mg protein (biuret), ammonium sulfate suspension	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£115.89 (exc VAT) per EACH	- +