LYS05

LYS05

Application

Lys05 has been used as an autophagy inhibitor to study its interactions with piperazine. It has also been used as a lysosomal inhibitor to study its effects on myogenesis in Gallus gallus.

Biochem/physiol Actions

Lys05 (Lys01 trihydrochloride) is a dimeric chloroquine (CQ) that deacidifies the lysosome and causes impairment of lysosomal enzymes, exhibiting more than 10-fold higher autophagy inhibitory potency than hydroxychloroquine (HCQ) and CQ (LC3II/LC3I ratio = 15.4 post 4 hr 10 µM treatment in LN229 glioblastoma cells vs. ﹤6.4 with 100 µM CQ or HCQ). Lys05 also shows higher anticancer activity both in vitro (IC50 3.6-6.0 µM vs. 15.6-23.8 µM with HCQ in 1205Lu, c8161, LN229, HT-29 cultures) and in mice in vivo (EC50 in reducing tumor growth rate ~10 mg/kg/day i.p.; HT29 xenografts) with toxicity observed only at high doses (≥80 mg/kg) as a result of significant lysozyme reduction.

No detailed specifications are available for this product.

There are no downloads for this product.

Code Description Colour Manufacturer Storage Temp. Solubility Form Assay Price Quantity
3575814 LYS05 white to beige SIGMA-ALDRICH −20°C DMSO: 2 mg/mL, clear
£490.29 (exc VAT) per 25MG
-
+
3575815 LYS05 white to beige SIGMA-ALDRICH −20°C DMSO: 2 mg/mL, clear
£142.07 (exc VAT) per 5MG
-
+