CellSplit® is a completely xeno-free, direct replacement to porcine or bovine-derived trypsin. It has been developed so that you can use the same protocol, eliminating any disruption to your current procedure. CellSplit® is suitable for both serum-free and serum-containing cell culture protocols due to the inactivation by dilution. Unlike trypsin, there is no requirement for specific inhibitors, such as foetal bovine serum (FBS). The active ingredient is of plant origin and manufactured to GMP grade. A cysteine protease that has broad specificity, cleaving the peptide bonds of leucine and glycine. Cysteine proteases have been extensively used for cell and tissue disassociation. CellSplit® has been comprehensively tested with a wide range of cell lines including Vero, HEK, C2, C12, L929, MRC5, LLCPK1, MDCK and CHO. It has also been tested with primary canine stem cells.
Completely xeno-free
Not derived from bacterial cultures
Plant-derived enzyme manufactured to GMP
UK CA and CE marked
Simple protocol
Inactivation by dilution, no need to use inactivation reagents such as FBS