Lipase from Rhizopus oryzae, powder (fine), approx. 10 U/mg

Lipase from Rhizopus oryzae, powder (fine), approx. 10 U/mg

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Rhizopus oryzae has been used:to test its effect on 1,2-diolein synthesis and triolein ethanolysisfor immobilization on graphene oxide support for biocatalysis studiesto digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis. ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

General description

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

Other Notes

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 µmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40°C

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3578856 Lipase from Rhizopus oryzae, powder (fine), approx. 10 U/mg 100 SIGMA-ALDRICH 2-8°C powder (fine)
£119.79 (exc VAT) per 1G
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3578857 Lipase from Rhizopus oryzae, powder (fine), approx. 10 U/mg 100 SIGMA-ALDRICH 2-8°C powder (fine)
£474.96 (exc VAT) per 5G
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