Lectin from Ulex europaeus (gorse, furze), lyophilized powder

Lectin from Ulex europaeus (gorse, furze), lyophilized powder

Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Linkage

Conjugates are prepared from affinity purified lectin.

Packaging

2, 5 mg in glass bottle

Preparation Note

Purified by affinity chromatography using the method of Pereira.

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Code Description Quality Level Manufacturer Composition Storage Temp. Impurities Form Price Quantity
3587083 Lectin from Ulex europaeus (gorse, furze), lyophilized powder 200 SIGMA-ALDRICH Protein, ≥80% biuret 2-8°C
£286.93 (exc VAT) per 2MG
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3587084 Lectin from Ulex europaeus (gorse, furze), lyophilized powder 200 SIGMA-ALDRICH Protein, ≥80% biuret 2-8°C
£313.39 (exc VAT) per 5MG
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