Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), Isolectin B4 (BSI-B4), biotin conjug

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), Isolectin B4 (BSI-B4), biotin conjug

Analysis Note

Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for immunohistochemistry and immunofluorescence staining.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) agglutinates human blood group A and B cells. It is specific for ?GalNAc- and ?Gal. It acts as a reagent for the detection of this ?Gal epitope in biological materials. Lectin from Bandeiraea simplicifolia is used as a marker for xenoreactive antigen (?Gal1?3Gal) and cancer. Lectin plays an important role in fertilization, embryogenesis, inflammation, metastasis and host-parasite recognition.

General description

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes ?GalNAc and ?Gal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Packaging

0.2, 1 mg in serum bottle

Physical form

Contains citrate buffer salts and calcium chloride

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Code Description Quality Level Manufacturer Composition Storage Temp. Form Price Quantity
3587046 Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), Isolectin B4 (BSI-B4), biotin conjug 200 SIGMA-ALDRICH Protein, ~95% E1%/280 −20°C lyophilized powder
£146.25 (exc VAT) per EACH
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3587047 Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), Isolectin B4 (BSI-B4), biotin conjug 200 SIGMA-ALDRICH Protein, ~95% E1%/280 −20°C lyophilized powder
£509.79 (exc VAT) per 1MG
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