Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.
Application
Lectin from Arachis hypogaea (peanut) has been used:to detect Thomson-Friedenreich antigenas a component in PBS to study its effects on cell viabilityto study its ability to block the binding site
Biochem/physiol Actions
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Lectin from Arachis hypogaea (peanut) possess specificity towards ? -D-Gal(1-3)-D-galNAc residue of Thomson ? Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.
General description
Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.