Lactoperoxidase from bovine milk, lyophilized powder (essentially salt-free), >=200 units/mg protein

Analysis Note

Protein determined by Lowry method.

Application

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H2O2 in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

Biochem/physiol Actions

Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

General description

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

Packaging

10, 25 mg in poly bottle

1, 5 mg in glass bottle

Unit Definition

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579765 Lactoperoxidase from bovine milk, lyophilized powder (essentially salt-free), >=200 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder (essentially salt-free)
£325.93 (exc VAT) per 10MG
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3579766 Lactoperoxidase from bovine milk, lyophilized powder (essentially salt-free), >=200 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder (essentially salt-free)
£555.75 (exc VAT) per 25MG
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3579767 Lactoperoxidase from bovine milk, lyophilized powder (essentially salt-free), >=200 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder (essentially salt-free)
£158.79 (exc VAT) per 5MG
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