L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units

L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units

Analysis Note

Protein determined by biuret

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA).

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.

General description

L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold.

Packaging

12500 units in glass bottle

Physical form

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Unit Definition

One unit will reduce 1.0 µmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579749 L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units 200 SIGMA-ALDRICH 2-8°C buffered aqueous glycerol solution
£487.50 (exc VAT) per EACH
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+
3579750 L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units 200 SIGMA-ALDRICH 2-8°C buffered aqueous glycerol solution
£168.54 (exc VAT) per EACH
-
+
3579751 L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, >=400 units 200 SIGMA-ALDRICH 2-8°C buffered aqueous glycerol solution
£813.43 (exc VAT) per EACH
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+