L-Glutamic Dehydrogenase from bovine liver, Type III, lyophilized powder, >=20 units/mg protein

L-Glutamic Dehydrogenase from bovine liver, Type III, lyophilized powder, >=20 units/mg protein

Analysis Note

Protein determined by biuret

Application

L-Glutamic Dehydrogenase was used to catalyzes the conversion of isocitrate into a-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species. The bovine enzyme is characterized by three sets of properties: It has a reversible concentration-dependent association, producing higher molecular weight forms. Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates. Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Packaging

100, 500 mg in poly bottle

1 g in poly bottle

Package size based on protein content

Physical form

Contains citrate and potassium phoshate buffer salts.

Unit Definition

One unit will reduce 1.0 µmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579628 L-Glutamic Dehydrogenase from bovine liver, Type III, lyophilized powder, >=20 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder
£445.71 (exc VAT) per 100MG
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+
3579629 L-Glutamic Dehydrogenase from bovine liver, Type III, lyophilized powder, >=20 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder
Click Add to Quote for price and delivery
-
+
3579630 L-Glutamic Dehydrogenase from bovine liver, Type III, lyophilized powder, >=20 units/mg protein 200 SIGMA-ALDRICH −20°C lyophilized powder
Click Add to Quote for price and delivery
-
+