L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei

L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei

Analysis Note

Protein determined by biuret.

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into ?-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species. The bovine enzyme is characterized by three sets of properties: It has a reversible concentration-dependent association, producing higher molecular weight forms. Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates. Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Physical form

50% glycerol solution

Unit Definition

One unit will reduce 1.0 µmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3579513 L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei 200 SIGMA-ALDRICH 2-8°C glycerol solution (50%)
£364.93 (exc VAT) per 100MG
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3579514 L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei 200 SIGMA-ALDRICH 2-8°C glycerol solution (50%)
£104.19 (exc VAT) per 20MG
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3579515 L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei 200 SIGMA-ALDRICH 2-8°C glycerol solution (50%)
Click Add to Quote for price and delivery
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3579516 L-Glutamic Dehydrogenase from bovine liver, Type II, aqueous glycerol solution, >=35 units/mg protei 200 SIGMA-ALDRICH 2-8°C glycerol solution (50%)
£208.93 (exc VAT) per 50MG
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