L-012 SODIUM SALT

L-012 SODIUM SALT

Application

L-012 has been used as a probe in a bioluminescent assay for the determination of the reactive oxygen species (ROS) levels in the isolated mouse lungs.

Biochem/physiol Actions

L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminol, lucigenin and MCLA. Instead of reacting with superoxide anion (O2−) directly, L-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical form, which then reacts with oxygen (O2) to yield O2− and L-012 quinone (q-Lum). The O2− in turn reacts with the LumH· radical, leading eventually to an endoperoxide that decomposes to emit luminescence. To a less extend, H2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONS, L-012 is also commonly used for screening NADPH oxidase inhibitors.

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Code Description Colour Manufacturer Storage Temp. Solubility Form Assay Price Quantity
3576011 L-012 SODIUM SALT yellow to orange SIGMA-ALDRICH −20°C H2O: 2 mg/mL, clear
£643.50 (exc VAT) per 25MG
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3576012 L-012 SODIUM SALT yellow to orange SIGMA-ALDRICH −20°C H2O: 2 mg/mL, clear
£160.18 (exc VAT) per 5MG
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