KAPA2G ROBUST HS RM+DYE

KAPA2G ROBUST HS RM+DYE

Application

KAPA2G Robust HotStart® ReadyMix™ has been used in: PCRReal time RT-PCRNested Polymerase Chain Reaction (nPCR)Amplification of GC- or AT-rich templatesTemplates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanolCrude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cellsColony PCRThe KAPA2G Robust HotStart ReadyMix may be combined with KAPA Express Extract for fast and efficient DNA extraction and PCR. For more information, refer to the application notes for: Blood PCR Mammalian DNA barcoding Fish DNA barcoding FFPE PCR

Biochem/physiol Actions

KAPA2G Robust HotStart® DNA Polymerase generated DNA fragments have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. Like wild-type Taq, KAPA2G Robust HotStart has 5’;→3’; polymerase and 5’;→3’; exonuclease activities, but no 3’;→5’; exonuclease (proofreading) activity.

Features and Benefits

Efficient amplification of GC- and AT-rich targets Robust performance across a wide range of GC- and AT-rich templates Increased PCR success ratesImproved tolerance to common PCR inhibitors Efficient amplification from crude samples Higher yield and sensitivity per unit of enzymeUnrivalled performance in colony PCR Higher yields and improved consistency of PCR direct from E. coli and yeast cellsQuick Notes: KAPA2G Robust HotStart ReadyMix PCR Kits contain KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance. Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template. Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates. KAPA2G Robust HotStart ReadyMix contains 2 mM MgCl2 at 1X. KAPA2G Robust HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels. KAPA2G Robust HotStart ReadyMix is ideal for single-protocol PCR, i.e. amplification of fragments varying from 25-65% GC, up to 1 kb in size, using a single reaction setup and cycling protocol. Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

General description

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

KAPA2G Robust HotStart® ReadyMix™ is ideally suited for the amplification of DNA fragments.

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Code Description Quality Level Feature Storage Temp. Price Quantity
2796000 KAPA2G ROBUST HS RM+DYE (1.25ML) 100 hotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included −20°C
£126.05 (exc VAT) per EACH
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2796001 KAPA2G ROBUST HS RM+DYE (6.25ML) 100 hotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included −20°C
£479.14 (exc VAT) per EACH
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