KAPA2G ROBUST HS RM

KAPA2G ROBUST HS RM

Application

KAPA2G Robust HotStart® PCR Kit has been used in:PCR amplification of saliva samplesPCR amplification for direct sequencingNested PCRAmplification of GC- or AT-rich templatesTemplates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanolCrude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cellsColony PCRRT-PCR

Biochem/physiol Actions

KAPA2G Robust HotStart® DNA Polymerase generated DNA are similar to fragments generated with wild-type Taq DNA polymerase. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. It may be used in restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 5’;→3’; polymerase and 5’;→3’; exonuclease activities, but no 3’;→5’; exonuclease (proofreading) activity.

Features and Benefits

Efficient amplification of GC- and AT-rich targets Robust performance across a wide range of GC- and AT-rich templates Increased PCR success ratesImproved tolerance to common PCR inhibitors Efficient amplification from crude samples Higher yield and sensitivity per unit of enzymeUnrivalled performance in colony PCR Higher yields and improved consistency of PCR direct from E. coli and yeast cellsQuick Notes: KAPA2G Robust HotStart PCR Kits contain KAPA2G Robust HotStart DNA Polymerase, engineered for high processivity and inhibitor tolerance. Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template. Use 0.5 U of KAPA2G Robust HotStart DNA Polymerase per 25 µL reaction. More challenging PCRs (GCrich,crude sample) may require higher enzyme concentrations. Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates. KAPA2G Buffers contain 1.5 mM MgCl2 at 1X. Additional MgCl2 may be added using the 25 mM MgCl2 solution provided in the kit. KAPA2G Buffer A is optimized for high yield, specificity, and sensitivity. KAPA2G Buffer B is recommended for inhibitorcontaminated template samples. KAPA2G GC Buffer is recommended for GC-rich PCR (>70% GC). KAPA Enhancer 1 can be used with KAPA2G Buffer A or B, particularly for GC-rich assays. Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

General description

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). KAPA2G Robust HotStart PCR Kit enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

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Code Description Quality Level Technique(s) Feature Storage Temp. Price Quantity
2795984 KAPA2G ROBUST HS (500U) 100 PCR: suitable hotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included: no −20°C
£403.93 (exc VAT) per EACH
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2795998 KAPA2G ROBUST HS RM (1.25ML) 100 - hotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included −20°C
£126.05 (exc VAT) per EACH
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2795999 KAPA2G ROBUST HS RM (6.25ML) 100 - hotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included −20°C
£479.14 (exc VAT) per EACH
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