JumpStart Taq ReadyMix for Quantitative PCR, For probe-based real-time PCR

Descriptions

Application

JumpStart^™ Taq ReadyMix^™ for Quantitative PCR has been used:for quantitative polymerase chain reaction (qPCR) amplification of HIV-1 RNA that is reverse transcribed and DNA using probes in a 2-step RT-qPCR assay for the quantification of reverse transcribed RNA in a study to monitor the activity of licorice in Treg cell differentiation and functionas a component of the reaction mixture for the detection and amplification of Salmonella sp. and internal amplification control (IAC) pUC 19 plasmid DNAas a component of the reaction mixture for the detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)for quantitative real-time -PCR of reverse-transcribed ß-arrestin-1 (ARRB1) and ?-arrestin-2 (ARRB2) mRNA

Features and Benefits

Ultimate Convenience: ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template Greater Specificity & Increased Target Yield: JumpStart^™ Taq prevents non-specific amplification and increased target yield Maximum Flexibility: Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR^® Green I Designed for use with either plate/tube real-time thermal cyclers or capillary instruments

General description

JumpStart^™ Taq ReadyMix^™ is a ready-to-use 2X master mix that contains JumpStart^™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart^™ Taq antibody. It combines the performance enhancements of our JumpStart^™ Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. Since it has no added dyes, this is the ideal solution for performing high-throughput, quantitative PCR methods that rely on a fluorescent probe. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Legal Information

SYBR is a registered trademark of Life Technologies

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

A license to perform the patented 5′ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5′ Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5′ Nuclease Kit, or (iii) license rights from Applied Biosystems. This product is an Authorized 5′ Nuclease Core Kit. Use of this product is covered by one or more of the following claims outside the US corresponding to US Patent No. 5,210,015 and 5,487,972. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research

Specifications

No detailed specifications are available for this product.

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Code	Description	Colour	Manufacturer	Compatibility	Feature	Form	Price	Quantity
3582260	JumpStart Taq ReadyMix for Quantitative PCR, For probe-based real-time PCR	colorless	SIGMA-ALDRICH	for use with Roche LightCycler 480, for use with ABI 7700, for use with Bio-Rad MJ Opticon 2, for use with Eppendorf® Mastercycler ep realplex, Bio-Rad iCy	hotstart, dNTPs included, Multiplex PCR	liquid	£261.86 (exc VAT) per 100RXN	- +
3582261	JumpStart Taq ReadyMix for Quantitative PCR, For probe-based real-time PCR	colorless	SIGMA-ALDRICH	for use with Roche LightCycler 480, for use with ABI 7700, for use with Bio-Rad MJ Opticon 2, for use with Eppendorf® Mastercycler ep realplex, Bio-Rad iCy	hotstart, dNTPs included, Multiplex PCR	liquid	£75.77 (exc VAT) per 20RXN	- +
3582262	JumpStart Taq ReadyMix for Quantitative PCR, For probe-based real-time PCR	colorless	SIGMA-ALDRICH	for use with Roche LightCycler 480, for use with ABI 7700, for use with Bio-Rad MJ Opticon 2, for use with Eppendorf® Mastercycler ep realplex, Bio-Rad iCy	hotstart, dNTPs included, Multiplex PCR	liquid	£705.00 (exc VAT) per 400RXN	- +