JumpStart Taq ReadyMix for High Throughput Quantitative PCR, Ready-to-use 2x mix for qPCR with ROX

JumpStart Taq ReadyMix for High Throughput Quantitative PCR, Ready-to-use 2x mix for qPCR with ROX

Application

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used: For quantitative polymerase chain reaction (qPCR) of cDNA templateAs a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytesFor qPCR amplifications using SYBRFor multiplex PCRFor reduction of primer dimers

Features and Benefits

JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time?and the risk of contamination from multiple pipetting steps This master mix allows consistent batch-to-batch and reaction-to-reaction performance Internal Reference Dye is provided for reaction normalization

General description

JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.To prepare a typical qPCR reaction, mix 25 µL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 µL. Reaction volumes can be scaled down, if desired.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Eppendorf is a registered trademark of Eppendorf AG

Packaging

Default reaction volume is 50 µL20RXN is packaged as 1 X 500 µL400RXN is packaged as 1 X 10 mL2000RXN is packaged as 1 X 50 mL

Quantity

Use 2.5 units per reaction.

No detailed specifications are available for this product.

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Code Description Colour Manufacturer Compatibility Feature Form Price Quantity
3582252 JumpStart Taq ReadyMix for High Throughput Quantitative PCR, Ready-to-use 2x mix for qPCR with ROX colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
Click Add to Quote for price and delivery
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3582253 JumpStart Taq ReadyMix for High Throughput Quantitative PCR, Ready-to-use 2x mix for qPCR with ROX colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
£81.03 (exc VAT) per 20RXN
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3582254 JumpStart Taq ReadyMix for High Throughput Quantitative PCR, Ready-to-use 2x mix for qPCR with ROX colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
Click Add to Quote for price and delivery
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+