Hybridization Solution, for Northern and Southern blots

Application

Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.

Components

Sigma’;s Hybridization Solution contains 200 µg/mL DNA in 10x SSC and 2X Denhardt’;s solutions:200 µg/mL DNA2mg Bovine Serum Albumin (BSA)2mg Ficoll2mg Polyvinylpyrrolidone (PVP)0.15 M sodium citrate1.5 M NaCl

General description

Sigma’;s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

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Code Description Quality Level Manufacturer Storage Temp. Form Price Quantity
3581950 Hybridization Solution, for Northern and Southern blots 100 SIGMA-ALDRICH −20°C liquid (2x concentrate)
£279.96 (exc VAT) per 50ML
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3581951 Hybridization Solution, for Northern and Southern blots 100 SIGMA-ALDRICH −20°C liquid (2x concentrate)
£945.75 (exc VAT) per EACH
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