Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, >=400 unit

Descriptions

Analysis Note

Protein determined by biuret.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C.dilatatafemales and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺ metalloenzyme.

Packaging

500, 1000, 2500 units in glass bottle

Physical form

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄ and 0.002 M KH₂PO₄ pH 6.5

Unit Definition

One unit will form 1.0?µmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH?6.6 at 25?°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579536	Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, >=400 unit	200	SIGMA-ALDRICH	2-8°C	buffered aqueous glycerol solution	£661.61 (exc VAT) per EACH	- +
3579537	Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, >=400 unit	200	SIGMA-ALDRICH	2-8°C	buffered aqueous glycerol solution	Click Add to Quote for price and delivery	- +
3579538	Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, >=400 unit	200	SIGMA-ALDRICH	2-8°C	buffered aqueous glycerol solution	£363.54 (exc VAT) per EACH	- +
3579539	Glyoxalase I from Saccharomyces cerevisiae, Grade IV, buffered aqueous glycerol solution, >=400 unit	200	SIGMA-ALDRICH	2-8°C	buffered aqueous glycerol solution	£331.50 (exc VAT) per EACH	- +