Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 un

Descriptions

Analysis Note

Protein determined by biuret.

Application

Glutamic-Oxalacetic Transaminase from porcine heart has been used:in an enzyme-coupled assay for evaluating L-asparaginase enzyme activityin microtiter plate format enzyme-based assay to estimate malic acid content in berry samplesas a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Biochem/physiol Actions

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and ?-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5?-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

General description

Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5?-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Packaging

10000 units in serum bottle

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

Unit Definition

One unit will convert 1.0 µmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579517	Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 un	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	Click Add to Quote for price and delivery	- +
3579518	Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 un	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£199.18 (exc VAT) per EACH	- +
3579519	Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 un	200	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£373.29 (exc VAT) per EACH	- +