Used as a selection agent (gentamicin-resistance gene) in molecular biology applications. It inhibits the growth of a wide variety of grampositive and gram-negative microorganisms including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.
Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis by binding to L6 protein of 50S ribosomal subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasma.
General description
Chemical structure: aminoglycoside
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Packaging
1g, 5g, 25g
Preparation Note
Freely soluble in water, practically insoluble in alcohol and ether.
Unit Definition
1 U corresponds to 1 µg of the USP gentamicin sulfate reference standard