GenElute#-E Blood DNA Kit

GenElute#-E Blood DNA Kit

Application

The GenElute™-E Single Spin Blood DNA Kit has been developed to purify genomic DNA from a wide variety of blood samples, including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used.The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods.?GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.

Store enzymes at 2-8°C for up to 12 months. All other components should be stored at room temperature.

Features and Benefits

Fast and efficient purification: 40-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers: Isolation of DNA in under an hour with minimal sample handling Better purity, leading to improved performance in PCR and other applications Significant reduction in plastic and hazardous chemical wasteTraditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.GenElute™-E kits employ size exclusion?negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and?plastic?materials required for purification.?This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures

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Code Description Manufacturer Feature Storage Temp. Price Quantity
3586842 GenElute#-E Blood DNA Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please 2-8°C
£65.05 (exc VAT) per EACH
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3586843 GenElute#-E Blood DNA Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please 2-8°C
£1,082.25 (exc VAT) per EACH
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3586844 GenElute#-E Blood DNA Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please 2-8°C
£253.50 (exc VAT) per EACH
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