Galactosyltransferase from bovine milk, lyophilized powder

Galactosyltransferase from bovine milk, lyophilized powder

Application

Galactosyltransferase from bovine milk has been used: in the generation of hyper-galactosylated variant of IgG1in ? -1,4-galactosyltransferase assay rice root cell extracts in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Biochem/physiol Actions

Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states.

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.

General description

Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Linkage

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Packaging

5, 10 units in serum bottle

25 units in glass bottle

Physical form

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Unit Definition

One unit will transfer 1.0?µmole of galactose from UDP-galactose to D-glucose per min at pH?8.4 at 30?°C in the presence of 0.2?mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

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Code Description Quality Level Manufacturer Composition Storage Temp. Impurities Form Price Quantity
3579555 Galactosyltransferase from bovine milk, lyophilized powder 200 SIGMA-ALDRICH Protein, 35-65% modified Warburg-Christian −20°C
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3579556 Galactosyltransferase from bovine milk, lyophilized powder 200 SIGMA-ALDRICH Protein, 35-65% modified Warburg-Christian −20°C
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3579557 Galactosyltransferase from bovine milk, lyophilized powder 200 SIGMA-ALDRICH Protein, 35-65% modified Warburg-Christian −20°C
£731.25 (exc VAT) per EACH
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