Ficin from fig tree latex, lyophilized powder

Descriptions

Application

Ficin from fig tree latex has been used: in the proteolysis of tendon collagen in the digestion of Chlorophyta Ulva rigida to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Ficin is effective in disrupting the staphylococcalbiofilms making it accessible antibiotics exposure.

General description

Extinction Coefficient: E^1% = 21.0 (280 nm)pI: 9.0

Packaging

100, 200, 1000 units in poly bottle

Physical form

Lyophilized powder containing L-cysteine and trace EDTA

Preparation Note

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Unit Definition

One unit will produce a δA₂₈₀ of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Storage Temp.	Form	Price	Quantity
3579447	Ficin from fig tree latex, lyophilized powder	200	SIGMA-ALDRICH	Protein, 80-100% biuret	−20°C	lyophilized powder	£186.64 (exc VAT) per EACH	- +
3579448	Ficin from fig tree latex, lyophilized powder	200	SIGMA-ALDRICH	Protein, 80-100% biuret	−20°C	lyophilized powder	Click Add to Quote for price and delivery	- +
3579449	Ficin from fig tree latex, lyophilized powder	200	SIGMA-ALDRICH	Protein, 80-100% biuret	−20°C	lyophilized powder	£341.25 (exc VAT) per EACH	- +