Fibronectin from rat plasma, powder, BioReagent, suitable for cell culture

Descriptions

Analysis Note

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Application

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.

Biochem/physiol Actions

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

General description

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Pack	Manufacturer	Product Line	Solubility	Form	Price	Quantity
3571642	Fibronectin from rat plasma, powder, BioReagent, suitable for cell culture	200	-	SIGMA-ALDRICH	BioReagent	H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)	powder	£555.00 (exc VAT) per EACH	- +
3571643	Fibronectin from rat plasma, powder, BioReagent, suitable for cell culture	200	1MG	SIGMA-ALDRICH	BioReagent	H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)	powder	£983.75 (exc VAT) per 1MG	- +
3571644	Fibronectin from rat plasma, powder, BioReagent, suitable for cell culture	200	2MG	SIGMA-ALDRICH	BioReagent	H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)	powder	Click Add to Quote for price and delivery	- +