Ethidium bromide, approx.95% (HPLC)

Ethidium bromide, approx.95% (HPLC)

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10?µg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.Frameshift mutagen which intercalates double-stranded DNA and RNA.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Packaging

1, 5, 10, 25 g in glass bottle

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 µg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 µg/ml and visualized immediately after electrophoresis.

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Code Description Quality Level Pack mp InChI key InChI Manufacturer Assay Price Quantity
3577422 Ethidium bromide, approx.95% (HPLC) 200 10G 260-262 °C (dec.) (lit.) ZMMJGEGLRURXTF-UHFFFAOYSA-N 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H SIGMA-ALDRICH ~95% (HPLC)
£368.68 (exc VAT) per 10G
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3577423 Ethidium bromide, approx.95% (HPLC) 200 1G 260-262 °C (dec.) (lit.) ZMMJGEGLRURXTF-UHFFFAOYSA-N 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H SIGMA-ALDRICH ~95% (HPLC)
£53.70 (exc VAT) per 1G
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3577424 Ethidium bromide, approx.95% (HPLC) 200 25G 260-262 °C (dec.) (lit.) ZMMJGEGLRURXTF-UHFFFAOYSA-N 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H SIGMA-ALDRICH ~95% (HPLC)
£654.32 (exc VAT) per 25G
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3577425 Ethidium bromide, approx.95% (HPLC) 200 5G 260-262 °C (dec.) (lit.) ZMMJGEGLRURXTF-UHFFFAOYSA-N 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H SIGMA-ALDRICH ~95% (HPLC)
£192.64 (exc VAT) per 5G
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