Esterase from porcine liver, ammonium sulfate suspension, >=150 units/mg protein (biuret)

Descriptions

Application

Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.

The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Biochem/physiol Actions

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.

General description

Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH 8

Unit Definition

One unit will hydrolyze 1.0 µmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579406	Esterase from porcine liver, ammonium sulfate suspension, >=150 units/mg protein (biuret)	300	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£72.15 (exc VAT) per EACH	- +
3579407	Esterase from porcine liver, ammonium sulfate suspension, >=150 units/mg protein (biuret)	300	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£45.96 (exc VAT) per EACH	- +
3579408	Esterase from porcine liver, ammonium sulfate suspension, >=150 units/mg protein (biuret)	300	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£807.86 (exc VAT) per EACH	- +
3579409	Esterase from porcine liver, ammonium sulfate suspension, >=150 units/mg protein (biuret)	300	SIGMA-ALDRICH	2-8°C	ammonium sulfate suspension	£243.75 (exc VAT) per EACH	- +