Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture

Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture

Application

Elastase from porcine pancreas is used for the following applications:Cell culture techniquesELISA techniquesUsed in assay for elastase inhibitory activity

Biochem/physiol Actions

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

General description

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Packaging

Package size based on protein content

1, 5 mg in glass bottle

20 mg in poly bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25°C.

No detailed specifications are available for this product.

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Code Description Quality Level Pack pH Manufacturer Form Price Quantity
3571591 Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture 200 1MG 8 SIGMA-ALDRICH
£47.75 (exc VAT) per 1MG
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3571592 Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture 200 20MG 8 SIGMA-ALDRICH
£547.50 (exc VAT) per 20MG
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3571593 Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture 200 5MG 8 SIGMA-ALDRICH
£163.75 (exc VAT) per 5MG
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