DL-Dithiothreitol, for electrophoresis, >=99%

DL-Dithiothreitol, for electrophoresis, >=99%

Analysis Note

Tested for use in reduced SDS-PAGE

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

1, 5, 25 g in poly bottle

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Code Description Quality Level Pack mp InChI key InChI Manufacturer Form Assay Price Quantity
3577419 DL-Dithiothreitol, for electrophoresis, >=99% 200 1G 40-44 °C, 41-44 °C (lit.) VHJLVAABSRFDPM-QWWZWVQMSA-N
£76.13 (exc VAT) per 1G
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3577420 DL-Dithiothreitol, for electrophoresis, >=99% 200 25G 40-44 °C, 41-44 °C (lit.) VHJLVAABSRFDPM-QWWZWVQMSA-N
£980.00 (exc VAT) per 25G
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3577421 DL-Dithiothreitol, for electrophoresis, >=99% 200 5G 40-44 °C, 41-44 °C (lit.) VHJLVAABSRFDPM-QWWZWVQMSA-N
£262.50 (exc VAT) per 5G
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