Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units

Descriptions

Analysis Note

Protein determined by biuret.

Application

Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.

DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus.

Used for the removal of DNA from protein samples.

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5?-phosphates. In the presence of Mg²⁺, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn²⁺. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn²⁺, Ca²⁺, Co²⁺, and Zn²⁺ are activators of the enzyme. A concentration of 5 mM Ca²⁺ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Packaging

20000, 40000, 200000, 500000 units in poly bottle

10000 units in glass bottle

Physical form

Contains calcium chloride

Preparation Note

The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose ﹤10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in ﹤10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.

Unit Definition

One Kunitz unit will produce a change in A₂₆₀ of 0.001 per minute per ml at pH 5.0 at 25 °C using DNA, Type I or III, as the substrate.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Form	Price	Quantity
3579343	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units	300	SIGMA-ALDRICH	Protein, ≥80%	lyophilized powder	£114.21 (exc VAT) per EACH	- +
3579344	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units	300	SIGMA-ALDRICH	Protein, ≥80%	lyophilized powder	Click Add to Quote for price and delivery	- +
3579345	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units	300	SIGMA-ALDRICH	Protein, ≥80%	lyophilized powder	£236.79 (exc VAT) per EACH	- +
3579346	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units	300	SIGMA-ALDRICH	Protein, ≥80%	lyophilized powder	£401.14 (exc VAT) per EACH	- +
3579347	Deoxyribonuclease I from bovine pancreas, Type II, lyophilized powder, Protein >=80 %, >=2,000 units	300	SIGMA-ALDRICH	Protein, ≥80%	lyophilized powder	Click Add to Quote for price and delivery	- +