Concanavalin A from Canavalia ensiformis (Jack bean), Sepharose conjugate, buffered aqueous suspensi

Descriptions

Analysis Note

Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Used for the purification of glycoproteins containing mannose.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Legal Information

Sepharose is a registered trademark of Cytiva

Packaging

25, 100 mL in glass bottle

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl₂ , MgCl₂, and MnCl₂

Specifications

No detailed specifications are available for this product.

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Code	Description	Capacity	Manufacturer	Form	Price		Quantity
3586708	Concanavalin A from Canavalia ensiformis (Jack bean), Sepharose conjugate, buffered aqueous suspensi	≥25 mg/mL binding capacity (thyroglobulin)	SIGMA-ALDRICH	buffered aqueous suspension	Click Add to Quote for price and delivery		- +
3586709	Concanavalin A from Canavalia ensiformis (Jack bean), Sepharose conjugate, buffered aqueous suspensi	≥25 mg/mL binding capacity (thyroglobulin)	SIGMA-ALDRICH	buffered aqueous suspension	£551.57 (exc VAT) per 25ML		- +