Carboxypeptidase B from porcine pancreas, lyophilized powder

Carboxypeptidase B from porcine pancreas, lyophilized powder

Analysis Note

Protein determined by biuret.

Application

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2’;-dipyridyl.

Packaging

Package size based on protein content

1, 5 mg in glass bottle

Physical form

Contains HEPES buffer salts, zinc chloride and carbohydrate

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Unit Definition

One unit will hydrolyze 1.0 µmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

No detailed specifications are available for this product.

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Code Description InChI key InChI Manufacturer Composition Form Price Quantity
3579312 Carboxypeptidase B from porcine pancreas, lyophilized powder TWURVFFNODFJBJ-UHFFFAOYSA-N 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20- SIGMA-ALDRICH Protein, 40-70% lyophilized powder
£105.16 (exc VAT) per 1MG
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3579313 Carboxypeptidase B from porcine pancreas, lyophilized powder TWURVFFNODFJBJ-UHFFFAOYSA-N 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20- SIGMA-ALDRICH Protein, 40-70% lyophilized powder
£459.64 (exc VAT) per 5MG
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