Betaine Solution, 5M, PCR Reagent, 1.5mL Vial

Betaine Solution, 5M, PCR Reagent, 1.5mL Vial

Application

Betaine solution has been used as a component of the reaction mixture for: loop-mediated isothermal amplification (LAMP) reactionspolymerase chain reaction for genomic DNA amplificationquantitative polymerase chain reaction (qPCR) for telomere length analysisin the PCR amplification of CGG repeats in the genomic DNAreverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)reverse transcription during library preparation using Smartseq2

General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘;long and accurate’; polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.

Packaging

1.5mL per vial

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Code Description Colour InChI key InChI Manufacturer Form Price Quantity
3582185 Betaine Solution, 5M, PCR Reagent, 1.5mL Vial colorless KWIUHFFTVRNATP-UHFFFAOYSA-N 1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3 SIGMA-ALDRICH liquid
£38.30 (exc VAT) per EACH
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3582186 Betaine Solution, 5M, PCR Reagent, 5x 1.5mL Vials colorless KWIUHFFTVRNATP-UHFFFAOYSA-N 1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3 SIGMA-ALDRICH liquid
£130.82 (exc VAT) per EACH
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