?-Lactoglobulin B from bovine milk has been used as: a protein standard in SDS-polyacrylamide electrophoresis for quantification of milk protein fractionsin the calibration of reversed phase- high-performance liquid chromatography (HPLC) for caseins quantificationfor immobilization on the biosensor surface and a calibration standards in biosensor assay
?-Lactoglobulin was used in the identification of the genetic variants of ?-casein in milk by isoelectric focusing electrophoresis.
Biochem/physiol Actions
?-Lactoglobulin B (?-LG B) show less inhibitory effect on the Staphylaococcus sp compared to ?-LG A.
General description
?-Lactoglobulin B (?-LG B), a isoform of ?-Lactoglobulin, is a small protein of 162 amino acids with a molecular mass of 18.2 kDa and optimum pH of 5.2. It is present in high level in casein and is relatively low in raw bovine milk. ?-LG has eight-stranded ?-barrel (strands A-H) succeeded by a three-turn ?-helix and a final ?-strand (strand I), that forms part of the dimerization interface.
Milk from dairy cows contains the protein ?-lactoglobulin (BLG). It naturally occurs in a number of genetic variants, and the most prevalent bovine variants are known as BLG A and BLG B.