beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL

Descriptions

Application

?-Glucuronidase from Helix pomatia has been used:to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detectionas a component in the protoplasting solutionfor the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples

?-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37?°C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37?°C.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price	Quantity
3579657	beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL	200	SIGMA-ALDRICH	2-8°C	aqueous solution	£320.36 (exc VAT) per 10ML	- +
3579658	beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL	200	SIGMA-ALDRICH	2-8°C	aqueous solution	£53.63 (exc VAT) per 1ML	- +
3579659	beta-Glucuronidase from Helix pomatia, Type H-3, aqueous solution, >=90,000 units/mL	200	SIGMA-ALDRICH	2-8°C	aqueous solution	£637.93 (exc VAT) per 25ML	- +